FGF-21 (FGF21) Human ELISA Kit

For the quantitative determination of human FGF-21 concentrations in serum, plasma and cell culture supernate samples.

Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions[1,2]. In murine models, FGF-21 is predominantly expressed in liver, but it also expressed in adipose tissue and pancreatic ß-cells[3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents[5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin, and glucagon[6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance[7].

[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635 [2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437 [3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206 [4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687– 26695 [5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35. [6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81 [7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.