IL-12

Symansis' IL-12^hcx contains the alpha and beta chains of human IL-12. It has N- and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatment with a glycosidase cocktail. Symansis' IL-12^hcx alpha chain contains 15-35% carbohydrate by weight. Symansis' IL-12^hcx beta chain contains 0-25% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Gately et al. (1998) Ann Rev Immunol 16:495-521