L-Selectin - Fc Chimera

Catalog Number: 7005
Availability: In Stock
Size: 10 µg, 25 µg, 50 µg
Uniprot Nu : P14151
Species Reactivity : Human
Host : Human, HEK Cell Expessed

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Symansis' purified L-Selectin - Fc Chimerahcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides. Symansis' L-Selectin - Fc Chimerahcx consists of 20-40% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Barkhausen T, et al. (2005) Exp Toxicol Pathol. 57: 39-52.
2. Fieger CB, et al. (2001) Biochim Biophys Acta 1524: 75-85.

 

 

 


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