IL-2 R α - Fc Chimera

Symansis' purified IL-2 R alpha - Fc Chimera^hcx has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis' IL-2 R alpha - Fc Chimera^hcx consists of 5-35% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' ^hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Waldmann TA (2002) J Clin Immunol. 22: 51-6. 2. Gaffen SL (2001) Cytokine 14: 63-77.