FGF R1 α (IIIc) - Fc Chimera

Symansis' FGF R1 alpha (IIIc) - Fc Chimera^hcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and probably O-linked oligosaccharides. Symansis' FGF R1 alpha (IIIc) - Fc Chimera^hcx contains 35-50% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Eswarakumar VP, et al. (2005) Cytokine Growth Factor Rev. 16: 139-49.
2. Ezzat S & Asa SL (2005) Horm Metab Res. 37: 355-60.