IL-5 R α (21-327 aa) - Fc Chimera/IL-5R α_Catalog 4202

Symansis' purified IL-5 R alpha (21-327 aa) - Fc Chimera^hcx   has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis' IL-5 R alpha (307 aa) - Fc Chimera^hcx consists of 5-30% carbohydrate by weight.  It is catalog number series 4202 and it consists of a Chimeric fursion protein containing aa 21-327 (307aa long) of the IL-5 Receptor alpha's extracellular domain fused to the Human IgG Fc domain (aa 93-300 of Human IgG Fc protein).

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Takatsu K (1998) Cytokine Growth Factor Rev. 9: 25-35.