EGF R - Fc Chimera

Catalog Number: 9021
Availability: In Stock
Size: 25 µg
Uniprot Nu : P00533
Species Reactivity : Human
Host : Human, HEK Cell Expessed

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Symansis' EGF R - Fchcx Chimera has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatement with a glycosidase cocktail. Symansis' EGF R - Fchcx Chimera contains 5-30% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Herbst RS (2004) Int. J. Radiation Oncology Biol. Phys., 59(2):21-26

 

 

 

Tags: 9021H, P00533


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