TrkB - Fc Chimera

Symansis' purified TrkB - Fc Chimera^hcx consists of 20-40% carbohydrate by weight. Symansis' TrkB - Fc Chimera^hcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Huang EJ & Reichardt LF (2003) Annu Rev Biochem. 72: 609-42.