IL-1 RI - Fc Chimera

IL-1RI is a glycoprotein expressed on T cells and fibroblasts. Glycosylation of IL-1RI has been shown to be necessary for optimal binding of IL-1.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

IL-1 is a proinflammatory cytokine involved in immune responses to infection, including both innate and acquired immunity and specifically stimulates T-helper cells to express cytokines associated with inflammatory responses. The functional effects of IL-1 are mediated through binding to the IL-1 receptors, of which three major receptors exist, namely IL-1RI, IL-1RII and IL-1R3.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Immunol Lett. (2005), 96(1):27-31