IL-1ra

Symansis' purified IL-1ra^hcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides. IL-1ra^hcx consists of 5-25% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Arend WP (2002) Cytokine Growth Factor Rev. 13: 323-40.