Flt-3 - Fc Chimera

Catalog Number: 9005
Availability: In Stock
Size: 25 µg
Uniprot Nu : P36888
Species Reactivity : Human
Host : Human, HEK Cell Expessed

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Symansis' Flt-3 - Fc Chimerahcx has N-linked and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Symansis' Flt-3 - Fc Chimerahcx contains 15-35% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Drexler HG & Quentmeier H (2004) Growth Factors 22: 71-3.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Tags: 9005F, 9005H, P36888


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