VEGF-C

Symansis' VEGF-C^hcx is the major secreted form, a disulfide linked antiparallel homodimeric protein with an observed molecular weight between 45 and 55 kDa. It has N- and probably O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatment with a glycosidase cocktail. Symansis' VEGF-C^hcx contains 15-40% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Ferrara N, et al. (2003) Nat Med. 9: 669-76.
2. Carmeliet (2005) Nature 438:932-936.