TNF RII - Fc Chimera, Carrier free formulation

Symansis' purified TNF RII - Fc Chimera^hcx has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides. Symansis' TNF RII - Fc Chimera^hcx consists of 5-35% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1. Carpentier I, et al. (2004) Curr Med Chem. 11: 2205-12.