CRC- β - Fc Chimera

Catalog Number: 1103
Availability: In Stock
Size: 25 µg, 50 µg
Uniprot Nu : P32927
Species Reactivity : Human
Host : Human, HEK Cell Expessed

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Symansis' purified CRC-beta - Fchcx Chimera has N- and O-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F and a further drop after treatment with a glycosidase cocktail. Symansis' CRC-beta - Fchcx Chimera consists of 0-25% carbohydrate by weight.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.

1. Scott CL and Begley CG (1999) Int J Biochem Cell Biol 31:1011-1015

 

 

 

Tags: 1103H, 1103I, P32927


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