CD33 - Fc Chimera/Siglec-3 - Fc Chimera
Availability: In Stock
Species Reactivity : Human
Host : Human, HEK Cell Expessed
Symansis' Siglec-3 - Fchcx Chimera has N-linked oligosaccharides, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F. Symansis' Siglec-3 - Fchcx Chimera contains 10-30% carbohydrate by weight.
Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.
1. Varki et al. (2006) Glycobiology 16:1R-27R