Growth Hormone R - Fc Chimera

Symansis' purified Growth Hormone R - Fc Chimera^hcx consists of 15-35% carbohydrate by weight. Symansis' Growth Hormone R - Fc Chimera^hcx has N-linked glycans, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides.

Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.

1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 

1. Strous GJ, et al. (2004) Curr Top Microbiol Immunol. 286: 81-118.