
Symansis supplies recombinant human proteins expressed from human cells (hcx) and ELISA kits with recombinant human protein standards expressed from human cells.
Symansis proteins are expressed from modified human 293 cells, which have the cellular machinery necessary to attach human post-translational modifications (PTMs) to the proteins being expressed. As such, they are different from recombinant human proteins expressed from other species.
Symansis utilises conventional chromatography purification techniques to purify the proteins. No tag-based affinity chromatography techniques are used for ligands. Each protein has purity greater than 95% by silver stain, with most proteins >97% pure.
|

Symansis antibodies are produced in sheep in the green fields of New Zealand.
Symansis reagents are all validated for use in their own right in the way described. The use of sheep antibodies also has the significant advantage by providing an alternative IgG species in experiments where more than one antibody are used. For example in immunoprecipitation experiments it means the problems with IgG band recognition can be avoided and it is very useful in western blotting techniques that simultaneously use more than one antibody (e.g. Dual labeling using LICOR technology)
|

Symansis offers ELISA kits with recombinant human protein standards expressed from human cells because species-to-species variation in post-translational modifications (PTMs) can affect ELISA immunoreactivity.
Research undertaken at Symansis has identified differences in immunoreactivity of human cytokines and growth factors expressed in human cells, compared to the same proteins expressed in non-human cells.
|